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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Clin Immunol. 2022 Dec 22;248:109213. doi: 10.1016/j.clim.2022.109213

Figure 8. Lupus nephritis patients’ serum activates iron uptake program and transcriptionally inhibits SLC7A11.

Figure 8.

While the serum iron was comparable between normal donors and lupus nephritis patients (A), only lupus nephritis serum rapidly (30 min) and significantly increased CD71 (TfR1), a major iron uptake protein in human proximal tubular cell line (HK-2 cells) (B-C). Dotted black line: Unstained cells; Light grey filled histogram: tissue culture medium treated cells; Red line: normal donor serum treated cells; Blue dotted line: Lupus nephritis serum treated cells. Compared to healthy controls, lupus nephritis serum attenuated the expression of the precursor mRNA and gene expression of SLC7A11, indicating transcriptional inhibition. Statistical significance was determined by 2-tailed Mann-Whitney test and using 2-way ANOVA with Tukey`s multiple comparison test. Only significant values are showed for comparison. Each dot represents an individual donor. Data is presented as mean. *P < 0.05, **P < 0.001, *** < 0.0001.