TABLE 1.

Electrotransformation efficiencies of plasmids in H. capsulatum G184ASura5-11^a

Plasmid	Size (kb)	Vector	Fungal insert	Physical state of transforming DNA	No. of expts	Transformation efficiency (log no./μg of DNA)
						Mean ± SD	Range
pWU1	6.5	pBR328	PaURA5 + 0.5 kb upstream and 0.4 kb downstreamb	Circular	4	0.2 ± 0.2	0.0–0.3
pWU3	4.5	pBLUESCRIPT	PaURA5 + 0.4 kb upstream and 0.4 kb downstreamb	Circular BamHI linearizedc	5 4	0.0 ± 0.0 2.0 ± 0.2	0.0 1.7–2.2
pWU44	6.7	pBR328	Same as pWU1	Circulard	5	2.3 ± 0.3	2.0–2.7
				HpaI linearizede	7	3.1 ± 0.2	3.0–3.6
pWU20	7.1	pBR328	HcURA5 + 0.4 kb upstream and 1.5 kb downstreamb	Circular	4	0.5 ± 0.4	0.0–1.0
pWU22	5.8	pBR328	HcURA5 + 0.1 kb upstream and 0.1 kb downstreamb	Circular EcoRV linearizedf	2 2	0.6 ± 0.1 2.2 ± 0.1	0.5–0.6 2.1–2.2
pWU51	6.3	pBR328	HcURA5 + 0.4 kb upstream and 0.1 kb downstreamb	CirculardHpaI linearizede	5 8	0.9 ± 0.2 3.0 ± 0.4	0.7–1.1 2.6–3.6

^aElectroporation conditions: 4 μg of circular plasmid or 1 μg of linearized plasmid as transforming DNA; 10% (wt/vol) mannitol in water as the electroporation solution; and electroporation with a capacitance of 25 μF, resistance of 600 Ω, and set voltage of 0.75 kV. We used a slightly larger amount (4 μg) of transforming DNA for circular plasmids because some showed no transformants with 1 μg. 

^bSequence flanking start and stop codons. 

^cNear downstream insert/vector junction. 

^dHaving adjacent, inverted telomeric repeat sequences. 

^eHaving telomeric termini. 

^fAt downstream insert/vector junction.