TABLE 3.

Electrotransformation of H. capsulatum with HpaI-linearized pMAD93

Expt	Strain	Time of transfer to hygromycin B plate after electroporation (h)a	Transformation efficiency (no. of transformants/ μg of DNA)
1	G184ASura5-11	—	640
		0	94
		6	580
		24	660
		48	690
2	G217Bura5-21	—	980
		0	<1
		6	3
		24	52
		48	140
3	G184ASura5-11	—	423
		0	15
		6	209
		24	312
		48	320

^aTransformed cells were continuously under uracil selection, i.e., on medium without uracil. —, never (only uracil selection).