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Structure of NAD+-bound CteC36–276.A, overall structure of NAD+-bound CteC36–276. mART domain and insertion domain are marked, with the electron density of NAD+ shown by Fo–Fc map (contour = 3σ). B, topology diagram of CteC36–276. C, interactions between NAD+ and CteC. D, activity assay probing crucial structural features affecting CteC activity. All reactions were performed using 50 nM and 200 nM CteC36–276, analyzed via native PAGE, and stained by Coomassie Blue. mART, mono-ART.
