Table 1.
Composition of culture medium.
| Culture media component | Concentration | Volume (mL) per 100 mL of media | Vendor |
|---|---|---|---|
| Dulbecco’s Modified Eagle Medium (DMEM)/F-12 HEPES, no phenol red | 93.3 | Thermo Fisher Scientific | |
| N-2 Supplement | 100× | 0.5 | Thermo Fisher Scientific |
| B-27™ Supplement | 50× | 1 | Thermo Fisher Scientific |
| 10% BSA in DPBS | 0.5 | MilliporeSigma | |
| 1-Thioglycerol | 11.5 M | 0.004 | MilliporeSigma |
| Ascorbic acid | 20 mg/mL | 0.25 | MilliporeSigma |
| Penicillin–streptomycin | 1 | Thermo Fisher Scientific | |
| GlutaMAX® Supplement | 1 | Thermo Fisher Scientific | |
| MEM Non-essential Amino Acids | 100× | 1 | Thermo Fisher Scientific |
| Insulin-Transferrin-Selenium | 100× | 1 | Thermo Fisher Scientific |
| Human Insulin Solution | 0.05 | MilliporeSigma | |
| Recombinant Human M-CSF Protein (rhM-CSF) | 100 μg/mL | 0.025 | PeproTech |
| Recombinant Human TGF beta 1 protein (rhTGFb1) | 100 μg/mL | 0.05 | R&D Systems |
| Recombinant Human IL-34 (rhIL-34) | 100 μg/mL | 0.1 | PeproTech |
| Recombinant human Fractalkine (rhFractalkine) | 100 μg/mL | 0.1 | PeproTech |
| Recombinant human CD200 (rhCD200) | 100 μg/mL | 0.1 | Acro Biosystems |