Fig. 4. bgcn-Cas9; kmo^sgRNAs split-drive can increase in frequency through a caged population.

Presence of kmo^sgRNAs and bgcn-Cas9 elements observed for 6 generations of small, caged populations and the percentages predicted by the deterministic (dashed lines) and stochastic (pale lines) models. Cages each generation beyond the initial setup were begun with 250 L1 larvae. a Initial frequencies for each cage at the outset of the trial. b Percentage of individuals carrying the kmo^sgRNAs transgene (blue solid lines) and/or the bgcn-Cas9 transgene (red solid lines). c Percentage of kmo^sgRNAs transgene in the absence of bgcn-Cas9 when female heterozygotes are crossed to either male heterozygotes (filled circles) or to wild type (WT) males (open circles). d Percentage of bgcn-Cas9 transgene in the absence of the kmo^sgRNAs, again with female heterozygotes crossed with male heterozygotes (filled circles) or to WT males (circles). The stochastic results show the behavior produced by each of 1000 independent numerical simulations. F cages were used to estimate the population size at each generation, details are included in Supplementary Dataset S1 and source data file.