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. 2023 Jul 3;26(2):311–325. doi: 10.1007/s12094-023-03258-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Zebrafish embryo anatomy and possible injection sites for GBM xenografting. Left: blastula period blastodisc allowing for injection into the cell mass or the yolk sac for the establishment of early embryonic xenograft models. Middle: brain regions of the developing zebrafish constituting possible injection sites for the establishment of late embryonic/larval orthotopic xenograft models. Right: alternative injection sites for orthotopic (brain ventricle) or heterotopic (duct of Cuvier, yolk sac, PVS) xenotransplantation. YSL yolk syncytial layer, PVS perivitelline space, SIV subintestinal vessels