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. 2023 Jul 3;26(2):311–325. doi: 10.1007/s12094-023-03258-7

Fig. 2.

Fig. 2

Zebrafish embryo anatomy and possible injection sites for GBM xenografting. Left: blastula period blastodisc allowing for injection into the cell mass or the yolk sac for the establishment of early embryonic xenograft models. Middle: brain regions of the developing zebrafish constituting possible injection sites for the establishment of late embryonic/larval orthotopic xenograft models. Right: alternative injection sites for orthotopic (brain ventricle) or heterotopic (duct of Cuvier, yolk sac, PVS) xenotransplantation. YSL yolk syncytial layer, PVS perivitelline space, SIV subintestinal vessels