TABLE 2.

Relative abundance of ceramides and related molecules in granulosa cells. Granulosa cells were collected from young mares (Yg, n = 5) and old mares (Old, n = 9) at 0 h (GV, germinal vesicle stage) or 24 h (MI, metaphase I stage) after maturation induction, with additional oocytes collected as 24 h and cultured in vitro for an additional 18 h (MIIC, metaphase II stage after culture). Results are presented as mean ± SEM and correction factor of 10x (CF). p-values for the main effect of stage (MS) and the interaction (INT) of stage-by-age are included in the table; the main effect of age was not significant (p > 0.05) for any of the metabolites. Different superscripts within a row represent differences at ^a,b,c p < 0.05 or ^d,e,f p < 0.1. Superscripts within a column for the same metabolite represent differences between Yg and Old at ^* p < 0.05 or ⁺ p < 0.1.

Ceramides and related molecules	Age	GV	MI	MIIC	CF	MS	INT
C18 Ceramide (d18:1/18:0)	Yg	10.81 ± 1.63	9.68 ± 1.59	6.47 ± 0.56	103	0.04	0.32
	Old	8.23 ± 1.10	10.61 ± 1.26	7.21 ± 1.31
Cer(d34:0)	Yg	1.34 ± 0.27	2.96 ± 0.48	2.61 ± 0.59	103	<0.001	0.40
	Old	1.19 ± 0.11a	3.99 ± 0.70b	2.99 ± 0.50c
Cer(d41:1)	Yg	2.58 ± 0.48ab	1.54 ± 0.25a,+	2.11 ± 0.35b	103	0.29	0.90
	Old	2.02 ± 0.23	2.33 ± 0.29+	2.78 ± 0.40
GalCer(d38:1)	Yg	15.44 ± 2.16a,d	8.80 ± 1.75ab,e	8.16 ± 1.09b	103	<0.001	0.73
	Old	14.05 ± 1.68a	9.83 ± 1.57	7.67 ± 0.85b
GalCer(d42:2)	Yg	11.24 ± 1.95a	5.06 ± 1.10b,*	5.59 ± 0.82ab	103	0.03	0.43
	Old	13.89 ± 3.05	10.51 ± 1.54*	12.85 ± 2.67
GlcCer(d34:1)	Yg	9.78 ± 2.12a	6.04 ± 1.83b	4.76 ± 0.60ab	103	<0.001	0.66
	Old	9.88 ± 1.70d	8.01 ± 0.99de	6.21 ± 1.22e

Cer, ceramide; GalCer, galactosylceramide; GlcCer, glucosylceramide.