Figure 5.

Effect of polyphenolic maqui extract in preventing NF-кB phosphorylation in macrophages cells in lamina propria from colons of Crohn’s disease-like colitis model. Immuno‐histochemistry analyses for NF-кB in 10 representative fields from colon tissue of each group (n= 6 mice/group): (A) Immune⁺-NF-кB staining intensity in macrophages cells (black arrow) in the control group, (B) CD group, (C) post-Treatment and (D) pre-Treatment groups. Immuno‐histochemistry analyses for p-NF-кB from 10 representative fields of view for each group: (F) Immune expression of p-NF-кB in macrophages cells in the control group (black arrow shows a single site in the field), (G) CD group, (H) post-Treatment group and (I) pre-Treatment group. Black arrows show positive staining in different sites in image (G–I). (E, J) Counting cells across 10 fields (400x magnification) for NF-кB and p-NF-кB expression in macrophages cells, respectively. Values represent mean ± SEM; *Difference at the p < 0.0005 level, ** Difference at the p < 0.0001 level; one-way ANOVA followed by Bonferroni’s Multiple Comparison test.