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Effect of varying TNF pretreatment times on the response of neutrophils to LPS (E. coli K-235). Cells were pretreated with 20 U of TNF/106 cells for 20 min and then with LPS (100 ng/ml) for 50 min. The results are derived from eight experiments with different donor cells. The results are expressed as stimulation indices (∗, P, <0.035; ∗∗, P < 0.015 [analysis of variance]).
