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. 2024 Jan 25;15(1):87. doi: 10.1038/s41419-024-06476-1

Fig. 4. Blocking AKT activation eliminates SHMT2-induced metastasis in thyroid cancer.

Fig. 4

A Protein levels of Vimentin were detected in BHP10-3 or IHH4 cells with SHMT2 overexpression and/or MK-2206 treatment by western blot, respectively. B Protein levels of Vimentin were examined in BHP10-3 or IHH4 cells with shSHMT2 and/or AKT transfection by western blot, respectively. C, D Migration assays were performed in BHP10-3 or IHH4 cells with SHMT2 overexpression and/or MK-2206 treatment, receptively. Left panel: representative micrographs of migrated cells. Scale bar: 50 μm. Right panel: statistical analysis of migrated cells. Data presented as mean ± SD, n = 8, n = 6. ***: p < 0.001, ns: no significant. E, F Migration assays were performed in BHP10-3 or IHH4 cells with shSHMT2 and/or AKT transfection. Left panel: representative micrographs of migrated cells. Scale bar: 50 μm. Right panel: statistical analysis of migrated cells. Data presented as mean ± SD, n = 6. **: p < 0.01, ***: p < 0.001, ns: no significant. G SHMT2 overexpressed and control BHP10-3 cells were injected in node mice via tail vein, followed by treatment with MK-2206 (5 mg/kg) for lung metastasis assays. Right panel: work follow of lung metastasis assays. Middle panel: representative histological micrographs of mice lung. Scale bar: 100 μm. Right panel: statistical analysis of metastasis tumor. Data presented as mean ± SD, n = 3. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ns: no significant.