Fig. 4. The autophagy-lysosome system plays a more important role in the degradation of hu-α-syn in both young and senescent glia than the ubiquitin-proteasome system.

A The glia were treated with hu-α-syn PFF for 1 h, washed out with PBS, replaced with medium containing MG132 (20 μM) or bafilomycin A1 (BafA1) (10 μM), and harvested at 1, 2, 6, 24 h following medium replacement. B Representative western blotting of remaining hu-α-syn in young and senescent glia with MG132 and BafA1 at each time point. C Quantification and statistical results of remaining hu-α-syn in young and senescent glia under treatment of MG132 or BafA1 (n = 3). Values are presented as means ± SEMs and statistical significance was determined by two-way ANOVA with the Bonferroni test. *p < 0.05, **p < 0.01.