Figure 2.

Nonspecific cellular transduction in floxed mouse brain by intravenously administered PHP.eB expressing Cre by mGfaABC1D promoter

(A) Schema showing experimental procedure. An Ai14 reporter mouse, which has the CAG promoter, bilaterally loxP-flanked STOP cassette, and cDNA of tdTomato, a red fluorescent protein, received intravenous injection of PHP.eB expressing Cre by mGfaABC1D promoter. (B) Immunohistochemistry of mouse brain 3 weeks after AAV injection (100 μL, 1 × 10¹³ vg/mL). Left, sagittal brain section immunostained for S100B. Right, magnification of a square region at left; tdTomato expression was too strong to distinguish individual cells. (C) Immunohistochemistry of cerebral cortex from mice that were similarly treated with (B), except for injection dose of AAV. Those mice received lower doses of AAV ranging from one-fifth to one-one hundredth. (D) Graphs showing specificity (Spe., left) and efficiency (Eff., right) for transduction of astrocytes and neurons. Data (average ±SD) were obtained from 4 mice, and the value from each mouse was plotted. n.s., not significant and ∗p < 0.05 by 1-way ANOVA with Bonferroni’s post hoc test. Scale bars, 100 μm.