Figure 3.

Significant upregulation of astrocyte transduction by shifting AAV capsid from PHP.eB to AAV-F

(A) Schema showing experimental procedure. Ai14 reporter mouse received intravenous injection of PHP.eB or AAV-F expressing Cre by mGfaABC1D promoter (100 μL, 4 × 10¹¹ vg/mL). (B) Immunohistochemistry of mouse brain 3 weeks after AAV injection. Astrocytes were immunolabeled for S100B. Fluorescence images of primary motor cortex from PHP.eB-treated (top) and AAV-F-treated (bottom) mice were presented, in which square regions were enlarged to present at respective upper right corners. Arrowheads indicate S100B-immunolabeled astrocytes. Scale bar, 100 μm. (C) Graph showing specificity (Spe.) and efficiency (Eff.) for astrocyte transduction (left) and that for neuronal transduction (right) in mice treated with PHP.eB or AAV-F vectors. Data (average ± SD) were obtained from 4 mice, and the value from each mouse was plotted. n.s., not significant; ∗p < 0.05; and ∗∗∗∗p < 0.0001 by unpaired Student’s t test.