Figure 5.

Astrocyte-specific transduction by intravenous infusion of 2 AAV-F mixtures expressing dCre in the presence of FlpO

(A) Schema showing experimental procedure. AAV genomes, which were the same as those in Figure 4, were coated with BBB-penetrating AAV-F capsid, instead of PHP.eB. AAV-F mixture (5 × 10¹¹ vg/mouse, respectively) was injected into Ai14 mice through the orbital plexus. (B) Immunohistochemistry of mouse brain 3 weeks after the AAV injection. A square region in the tdTomato fluorescence image of sagittal brain section (top) was magnified (bottom), in which square regions were enlarged and presented at upper right corners. Arrowheads indicate S100B-labeled astrocytes. Scale bar, 100 μm. (C and D) Graph showing specificity (C) and efficiency (D) for astrocyte and neuronal transduction. Data (average ± SD) were obtained from 4 mice, and the value from each mouse was plotted.