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. 2023 Nov 27;11(4):2305442. doi: 10.1002/advs.202305442

Figure 3.

Figure 3

cGas‐Sting signaling pathway was activated in LPS‐induced microglia. a) Western blot and quantitative analysis of cGas and Sting in LPS‐induced microglia of 0, 1, 5, and 10 µg mL−1 after 24 h. b) Western blot analysis of cGas, Sting, Irf3, p‐Irf3, P65, and p‐P65 protein expression in LPS (5 µg mL−1)‐induced microglia. c) Representative of immunofluorescence staining of Sting in microglia (Scale bar: 20 µm). d) Western blot and quantitative analysis of cGas, Sting, Irf3, p‐Irf3, P65, and p‐P65 in microglia of Control, LPS, LPS + vehicle, and LPS + C‐176. e) Representative immunostained images of M1 state (iNOS) microglia. f) Levels of pro‐inflammatory cytokines, including IL‐1β, IFN‐β, and TNF‐α in the microglia medium. g) Flow cytometric analysis on the expression levels of M1 microglia ratio (F4/80/ CD86+). n = 6 for each group. Error bars denote mean ± SEM, ns, no significance, *p < 0.05, **p < 0.01, and ***p < 0.001.