Figure 4.

Sting signaling activation in LPS‐induced microglia enhanced neuron death. a) Western blot analysis of cleaved‐Parp expression in the perilesional tissues at 7 d after SCI. b) NeuN/TUNEL double immunostaining in the perilesional tissues 7 d after SCI (Scale bar: 50 µm). c) The protocol of in vitro experiments for detecting neurons (CATH.a) death regulated by Sting activation in primary microglia (by Figdraw). Primary microglia treated with PBS or LPS (5 µg mL⁻¹) for 24 h. After removal of the supernatant, cells were cultured with DMEM culture medium for 24 h, and then the supernatant was collected as neurons conditioned medium for 24 h. d) Western blot analysis of cleaved‐Parp expression in neurons in each group. e) The neuron death rate was measured by Annexin V and PI staining. n = 6 for each group. Error bars denote mean ± SEM, ns, no significance, ^* p < 0.05, ^** p < 0.01, and ^*** p < 0.001.