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. 2024 Jan 23;15(1):20220330. doi: 10.1515/tnsci-2022-0330

Table 1.

Primer sequence information for SCN1A, SCN1A-dsAS, and SCN1A-usAS

Target Primer 5′→ 3′ sequence
TBP TBP-fw CGGTTTGCTGCGGTAATCAT
TBP-rv GGTGAGCACAAGGCCTTCTA
SCN1A SCN1A-fw GCTGTATACATCCGTGTGCAG
SCN1A-rv GCGTCTTTCAATAGCCGCAA
SCN1A-dsAS SCN1A-dsAS-fw CCTTGTACACCAGGGCATTC
SCN1A-dsAS-rv GTGGTATAGGAACTGGCAGCA
SCN1A-usAS SCN1A-usAS-fw CCAGGAAACAGGAATTCAGGC
SCN1A-usAS-rv CGAGTGATCCGTCTTGCCA

fw = forward, rv = reverse, TBP (housekeeping) = TATA box binding protein, SCN1A = sodium channel 1 α subunit, SCN1A-dsAS = SCN1A downstream antisense transcript: NR_110598, SCN1A-usAS = SCN1A upstream antisense transcript: NR_110260.