Figure 4.

Substrate range tests for the “Ca. A. nitratireducens” enriched in the C₂H₆-, C₃H₈-, and C₄H₁₀-fed bioreactors; subculture from the ethane bioreactor supplemented with propane (A) or butane (B) showed simultaneous nitrate and propane/butane consumption with production of dinitrogen gas and ammonium; the same was observed for subcultures from the propane bioreactor supplemented with ethane (C) and butane (D), and the butane bioreactor provided with ethane (E) and propane (F); each test was conducted in triplicate (results of other tests were included in Supplementary Fig. 11).