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. 2024 Jan 10;18(1):wrad013. doi: 10.1093/ismejo/wrad013

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© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Microbial community successions on brown macroalgal leachate; (A) experimental setup for studying the succession of natural microbial communities on leachate of the brown seaweed F. vesiculosus (FL); left: map of Nahant [58] showing the sampling locations of the seawater and sediment communities used as inocula (scale: 1:10 000); right: schematic of the self-assembly process on FL; inoculant communities were grown in FL batch media for 216 h, and at each time point between 6 and 216 h, cell count and DOC measurements were taken; community composition was determined in the inocula and at each time point by 16S rRNA gene tag sequencing; ~30–40 isolates were collected from each replicate community at each time point; (B) average trajectory of total bacterial abundances (as cell density) and the change in concentration of DOC relative to t = 6 h (negative values = decrease of DOC) in the seawater- and sediment-inoculated communities over time; error bars are standard deviations (n = 3); (C) community composition over time shown as the relative abundances of taxa averaged over three replicates from seawater- and sediment-inoculated communities; the top 40 most abundant taxa (amplified sequence variants, ASVs) are shown in color; the sum of other ASVs is shown in gray; the community composition changes from 0 to 6 h in the seawater-inoculated communities are in a separate panel on the left to emphasize the change from the inoculum to the first bloom; (D) distribution of the COM, ) for the absolute abundance of individual taxa in the seawater- (blue, left) and sediment- (brown, right) inoculated communities; distributions are represented as both histograms (colored bars) and kernel density estimations (black lines); absolute abundances are calculated as the product of community cell density in (B) with relative abundances in (C); gray lines at the first trough of density plots represents the cutoff between early and late growers in the successions; colored dots represent the five selected representative ASVs from early and late bloomers, respectively.

Inline graphic — Microbial community successions on brown macroalgal leachate; (A) experimental setup for studying the succession of natural microbial communities on leachate of the brown seaweed F. vesiculosus (FL); left: map of Nahant [58] showing the sampling locations of the seawater and sediment communities used as inocula (scale: 1:10 000); right: schematic of the self-assembly process on FL; inoculant communities were grown in FL batch media for 216 h, and at each time point between 6 and 216 h, cell count and DOC measurements were taken; community composition was determined in the inocula and at each time point by 16S rRNA gene tag sequencing; ~30–40 isolates were collected from each replicate community at each time point; (B) average trajectory of total bacterial abundances (as cell density) and the change in concentration of DOC relative to t = 6 h (negative values = decrease of DOC) in the seawater- and sediment-inoculated communities over time; error bars are standard deviations (n = 3); (C) community composition over time shown as the relative abundances of taxa averaged over three replicates from seawater- and sediment-inoculated communities; the top 40 most abundant taxa (amplified sequence variants, ASVs) are shown in color; the sum of other ASVs is shown in gray; the community composition changes from 0 to 6 h in the seawater-inoculated communities are in a separate panel on the left to emphasize the change from the inoculum to the first bloom; (D) distribution of the COM, ) for the absolute abundance of individual taxa in the seawater- (blue, left) and sediment- (brown, right) inoculated communities; distributions are represented as both histograms (colored bars) and kernel density estimations (black lines); absolute abundances are calculated as the product of community cell density in (B) with relative abundances in (C); gray lines at the first trough of density plots represents the cutoff between early and late growers in the successions; colored dots represent the five selected representative ASVs from early and late bloomers, respectively.