Figure 3.

Changes in immune cell populations in mice implanted with Clone M3 tumors. (A) Whole blood collected at end of study found changes in granulocytes, CD4+ T cells, macrophages and M1 macrophages. (B) Axial and inguinal lymph nodes collected at end of study found significant increases in NK cells. Samples were collected on Day 20 in animals left untreated (black bar) or administered 3 cycles of IT vehicle + IP isotype control (blue bar), IT LSAM-PTX (~60 mg/kg; green bar), IP anti-mPD-1 (10 mg/kg; Orange bar) or IT LSAM-PTX (~60 mg/kg) + IP anti-mPD-1 (10 mg/kg) combination treatment (red bar). For each population, data are displayed as group mean of %CD45+ cells + SEM. Comparison to IT Vehicle + IP isotype control performed using Kruskal–Wallis and Dunn’s multiple comparisons tests; * = p < 0.05; ** = p < 0.01.