TABLE 2.
Phenotypic and genotypic properties of 31 S. canis isolates from cows with subclinical mastitis in comparison with those of five S. canis control strains
| Property | Reactiona
|
|
|---|---|---|
| S. canis (n = 31) | S. canis control strains (n = 5) | |
| Growth on: | ||
| KAA | − | − |
| 6.5% NaCl | − | − |
| Beta-hemolysis | + | + |
| Carbohydrate fermentation | ||
| Glucose, maltose, ribose, saccharose | + | + |
| Inulin, lactose, mannitol, raffinose, salicin, sorbit, mannit, trehalose | − | − |
| Hydrolysis of: | ||
| Esculin | +b | + |
| Arginine | + | + |
| Sodium hippurate | − | − |
| Enzyme activity | ||
| α-Galactosidase | +c | + |
| β-Galactosidase | + | + |
| β-d-Glucuronidase | − | − |
| Pyrrolidonyl arylamidase in API 20 Strep system | − | − |
| CAMP-like factor on sheep blood agar plates | + | + |
| Specific PCR | ||
| cfg gene | + | + |
| sodAint gene | + | + |
a
+, 100% positive reaction; −, 0% positive reaction.
b
Positive after second subculture.
c
Of the isolates tested, 83.9% were positive.