Table 1.
Antibodies and immunohistochemical protocols
| Antibody specificity | Clone | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
| Vimentin | Monoclonal (V9) | 1:200 | Heat (Dakocitomaker), 3 mins in pH 6.0 citrate buffer for 125°C | Zymed |
| Iba-1 | Polyclonal antibody (Wako no. 019-1974) | 1:1000 | Heat, 10 mins in pH 6.0 citrate buffer | Dako |
| Desmin | Monoclonal (D33) | 1:300 | Heat, 10 mins in pH 9.0 Tris EDTA | Dako |
| Smooth muscle actin | Monoclonal Clone 1A4 | 1:50 | Heat, 20 mins in pH 9.0 Tris EDTA | Dako |
| S-100 | Monoclonal (Code IR50461-2) | Ready to use | Heat, 10 mins in pH 6.0 citrate buffer | Dako |
Iba-1 = ionized calcium-binding adaptor molecule 1