Figure 6. Stellate BCAS1⁺ cells possess proliferate capacity.

(A) BCAS1 and KI67 double immunofluorescence reveal the capacity to proliferate of stellate BCAS1⁺ cells in OG and GB (yellow arrowheads). In AS, stellate BCAS1⁺ cells do not proliferate (white arrowhead). Spherical BCAS1⁺ cells do not proliferate in any diffuse glioma analyzed (white arrowheads). (B) BCAS1⁺ cells forming nodules also display a proliferative capacity (yellow arrowheads). (C) BCAS1⁺ cells do not proliferate in non-tumor brain tissue. (D) Proliferation rate of the BCAS1⁺ in grade 2 and grade 3 OG. (E) Proliferation rate of the BCAS1⁺ cells when comparing the isolated and nodule distribution pattern. For double immunofluorescence staining: n = 17 OG, n = 8 AS, n = 60 GB. For (d) quantification: n = 3 grade 2 OG, n = 6 grade 3 OG; Mann–Whitney test. Center lines in the boxes indicate the median and whiskers indicate the minimum and the maximum values. Abbreviation: NS: not significant. For (e) quantification: n = 4 OG with isolated BCAS1⁺ cells, n = 5 OG with BCAS1⁺ nodules; Mann–Whitney test. Center lines in the boxes indicate the median and whiskers indicate the minimum and the maximum values. Abbreviation: NS: not significant. Scale bars: A, B) 25 μm; C) 20 μm.