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hMPV-seropositive and hMPV-seronegative serum samples based on VSV-hMPV F recombinant ELISAs. VSV-wt lysate or VSV-hMPV F lysate was used as antigen in an ELISA of human serum. Serum specimens from subjects 002 (A) and 008 (B) were serially diluted and allowed to bind antigen. Binding was measured by optical densitometry following incubation with a conjugated secondary antibody and a chromogenic substrate.
