FIG. 2.

Autoradiogram of Southern blot analysis of HCMV PCR products. PCR products were separated on a 3% agarose gel, transferred to a nylon membrane, and hybridized with internal oligonucleotide P2 (21) end labeled by T4 polynucleotide kinase and [γ-³²P]ATP. Hybridization was carried out for 18 h at 52°C in 5× SSC-5× Denhardt's solution-0.1% sodium dodecyl sulfate. After washing, the filters were exposed to a phosphoscreen. Hybridization signals were visualized by phosphoimaging (PhosphorImager, type SI). P, positive control (0.1 pg of AD169 DNA); N, negative (buffer) control; M, breast milk; TA, tracheal aspirate; U, urine. The PCR products from one mother-child pair are underlined.