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. 2023 Dec 28;13(1):53. doi: 10.3390/antiox13010053

Figure 4.

Figure 4

The antioxidant effect of vitamin B12 on oocyte mitochondria and vitellogenin in CIA. (A) Comparison of mitochondrial morphology in the oocytes of transgenic animals (EGD623, egxSi152 [mex-5p:: tomm-20::gfp::pie-1]) fed with or without caffeine after NAC treatment. Bar, 5 μm. The graph shows the distribution of mitochondrial morphology in the −1 oocyte. (B) Representative images and graphs show the relative level of mitochondrial reactive oxygen species using CellROX Green dye in the oocytes of CIA with NAC treatment. Bar, 5 μm. (C) Comparison of the relative intensity of VIT-2::GFP in CIA supplemented with NAC or NAC + vitamin B12. Bar, 50 μm. (D) The graph shows the number of diakinesis oocytes in CIA supplemented with NAC or NAC + vitamin B12. Data represent mean ± standard deviation. *, p < 0.05. **, p < 0.01. ***, p < 0.001. Number of analyzed animals, n ≥ 20 in respective conditions.