Table 1.
Lipid peroxidation products, oxidized proteins and the main polyunsaturated fatty acids measured in the cerebellum of Sprague-Dawley rats fed either a standard (STD) or a high-fat and high-sucrose diet (HFHS), and a weekly dietary supplementation of either soybean oil (control) or fish oil (ω3) 1.
| STD-C | STD-ω3 | HFHS-C | HFHS-ω3 | |
|---|---|---|---|---|
| Conjugated dienes (mmoles hydroperoxide/kg lipid) | 6.30 a (1.37) | 5.64 a (1.77) | 6.79 a (0.32) | 6.54 a (1.21) |
| HNE (a.u./μg lipid) | 643.56 a (141.17) | 627.00 a (61.32) | 750.11 ab (69.12) | 764.89 b (85.04) |
| MDA (a.u./μg lipid) | 573.00 a (88.71) | 556.00 a (79.65) | 661.56 b (34.00) | 641.22 b (15.68) |
| OxPC (a.u./μg lipid) | 140.19 a (10.80) | 171.88 ab (16.81) | 199.48 b (10.01) | 170.23 ab (6.45) |
| HNE-protein adducts (a.u./μg protein) | 22.97 b (1.00) | 24.08 ab (3.20) | 25.40 a (1.35) | 20.53 c (0.78) |
| MDA-protein adducts (a.u./μg protein) | 20.45 ab (1.55) | 21.35 ab (2.14) | 22.781 a (3.01) | 18.05 b (1.35) |
| OxPC-protein adducts (a.u./μg protein) | 76.11 a (5.82) | 72.31 a (0.57) | 78.33 a (8.21) | 77.85 a (1.26) |
| Total protein carbonylation (a.u./μg protein) *# | 68.70.1 b (7.32) | 80.62 ab (12.63) | 100.01 a (9.16) | 84.01 ab (11.50) |
| Protein carbonylation of cytosolic proteins (a.u./μg protein) *# | 67.3 b (2.1) | 81.9 ab (14.4) | 104.7 a (24.4) | 79.40 b (20.6) |
| Protein carbonylation of myofibrillar proteins (a.u./μg protein) * | 70.1 b (12.5) | 79.3 ab (23.6) | 95.3 a (8.45) | 88.6 ab (5.60) |
| % ω6-PUFAs (% total fatty acids) *$ | 11.47 a (0.43) | 10.90 a (0.38) | 10.80 a (0.67) | 9.62 b (0.46) |
| % ω3-PUFAs (% total fatty acids) | 12.80 b (1.23) | 14.04 a (0.79) | 14.34 ab (1.47) | 14.21 ab (1.56) |
| ω3/ω6 *$ | 1.12 c (0.09) | 1.29 b (0.06) | 1.33 ab (0.09) | 1.48 a (0.10) |
| Total PUFAs (% total fatty acids) | 24.27 a (1.50) | 24.93 a (1.06) | 25.14 a (2.05) | 23.83 a (1.99) |
1 Two-way ANOVA analyses were conducted. * p < 0.05 significant differences given by the factor “diet” (STD and HFHS); $ p < 0.05 significant differences given by the factor “supplement” (CONTROL, ω-3). Superscript # indicates a significant interaction (p < 0.05) between the factors diet and supplement. Means with different superscript a–c indicate significant differences (p < 0.05) (analyzed by post hoc Tukey HSD).