. 2024 Jan 15;13(1):103. doi: 10.3390/antiox13010103

Table 2.

Proteins whose carbonylation level was modulated by the high-fat and high-sucrose diet, the fish oil supplementation or both in the cerebellum of Sprague–Dawley rats ¹.

				Specific Protein Carbonylation (a.u./μg Protein)
Spot Nº	Figure	Protein Abbreviation	Protein Description	STD-C	STD-ω3	HFHS-C	HFHS-ω3
2	S2	ACTB	Actin cytoplasmic 1 ^$	0.14 ^ab (0.06)	0.22 ^ab (0.06)	0.14 ^b (0.08)	0.28 ^a (0.05)
23	S1	AKR1A1	Aldo-keto reductase family 1 member A1 ^$#	0.30 ^a (0.09)	0.09 ^b (0.03)	0.16 ^ab (0.02)	0.13 ^b (0.07)
33	S1	ALDOC	Fructose-bisphosphate aldolase C *	0.16 ^a (0.06)	0.15 ^a (0.04)	0.26 ^a (0.08)	0.21 ^a (0.01)
4	S2	ATP5F1B	ATP synthase subunit beta mitochondrial *	0.05 ^a (0.02)	0.07 ^a (0.01)	0.11 ^a (0.04)	0.17 ^a (0.09)
15	S2	ATP5F1B	ATP synthase subunit beta mitochondrial ^$	0.17 ^ab (0.04)	0.14 ^ab (0.05)	0.24 ^a (0.06)	0.12 ^b (0.02)
33	S1	CA2	Carbonic anhydrase 2 *	0.16 ^a (0.06)	0.15 ^a (0.04)	0.26 ^a (0.08)	0.21 ^a (0.01)
3	S1	CKB	Creatine kinase B-type *	0.05 ^a (0.02)	0.07 ^a (0.01)	0.12 ^a (0.08)	0.11 ^a (0.04)
7	S2	CKB	Creatine kinase B-type ^$	0.30 ^ab (0.13)	0.22 ^ab (0.04)	0.43 ^a (0.09)	0.21 ^b (0.04)
30	S2	CKMT1	Creatine kinase U-type mitochondrial *^$#	0.08 ^b (0.02)	0.06 ^b (0.01)	0.14 ^a (0.02)	0.05 ^b (0.02)
8	S1	ENO1	Alpha-enolase *^$	0.02 ^b (0.00)	0.04 ^ab (0.02)	0.04 ^ab (0.01)	0.05 ^a (0.01)
24	S1	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase ^$	0.31 ^a (0.01)	0.15 ^b (0.07)	0.30 ^a (0.05)	0.13 ^b (0.04)
9	S2	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase ^$	0.21 ^a (0.06)	0.12 ^a (0.07)	0.25 ^a (0.05)	0.15 ^a (0.03)
10	S2	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase ^$	0.19 ^a (0.03)	0.05 ^b (0.01)	0.13 ^a (0.01)	0.11 ^ab (0.05)
6	S2	GLUD1	Glutamate dehydrogenase 1 mitochondrial ^$	0.45 ^a (0.13)	0.29 ^a (0.08)	0.45 ^a (0.09)	0.29 ^a (0.07)
17	S2	GLUD1	Glutamate dehydrogenase 1 mitochondrial ^$	0.24 ^a (0.12)	0.13 ^a (0.02)	0.19 ^a (0.03)	0.13 ^a (0.03)
7	S2	GLUL	Glutamine synthetase ^$	0.30 ^ab (0.13)	0.22 ^ab (0.04)	0.43 ^a (0.09)	0.21 ^b (0.04)
23	S2	GLUL	Glutamine synthetase ^$	0.16 ^a (0.04)	0.14 ^a (0.04)	0.21 ^a (0.07)	0.11 ^a (0.03)
5	S2	NEFL	Neurofilament light polypeptide ^#	0.20 ^b (0.07)	0.49 ^a (0.13)	0.31 ^ab (0.12)	0.25 ^ab (0.04)
5	S2	PACSIN1	Protein kinase C and casein kinase substrate in neurons protein 1 ^#	0.20 ^b (0.07)	0.49 ^a (0.13)	0.31 ^ab (0.12)	0.25 ^ab (0.04)
7	S2	PDHA1	Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial ^$	0.30 ^ab (0.13)	0.22 ^ab (0.04)	0.43 ^a (0.09)	0.21 ^b (0.07)
23	S2	PDHA1	Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial ^$	0.16 ^a (0.04)	0.14 ^a (0.04)	0.21 ^a (0.07)	0.11 ^a (0.03)
33	S1	PGAM1	Phosphoglycerate mutase 1 *	0.16 ^a (0.06)	0.15 ^a (0.04)	0.26 ^a (0.08)	0.21 ^a (0.01)
15	S2	SEPTIN11	Septin-11 ^$	0.17 ^ab (0.04)	0.14 ^ab (0.05)	0.24 ^a (0.06)	0.12 ^b (0.02)
23	S1	SYN1	Synapsin-1 ^$	0.30 ^a (0.09)	0.09 ^b (0.03)	0.16 ^ab (0.02)	0.13 ^b (0.07)
9	S2	SYN1	Synapsin-1 ^$	0.21 ^a (0.06)	0.12 ^a (0.07)	0.25 ^a (0.05)	0.15 ^a (0.03)

¹ Two-way ANOVA analyses were conducted. * p < 0.05 significant differences given by the factor “diet” (STD and HFHS); ^$ p < 0.05 significant differences given by the factor “supplement” (CONTROL, ω-3). Superscript ^# indicates a significant interaction (p < 0.05) between the factors diet and supplement. Means with different superscript ^a,b indicate significant differences (p < 0.05) (analyzed by post hoc Tukey HSD). Spot Nº refers to the numbered spots in 2-DE gel images shown in Figures S1 and S2. a.u.: arbitrary units.