Skip to main content
. 2023 Dec 25;13(1):42. doi: 10.3390/antiox13010042

Figure 6.

Figure 6

HSPA8 translocation to mitochondria to inhibit ALDH2 enzyme activity after OGD. (A) Co-IP-MS detection principle and workflow. (B) The top five proteins binding to ALDH2 under OGD conditions. (C) Subcellular fractionation of NIH3T3 cells treated with OGD for 4 h were immunoprecipitated using ALDH2 antibody, and bound proteins were analyzed using Western blot. (D) Western blot analysis of HSPA8 in NIH3T3 cells. (E) Quantification of (D). *** p < 0.001 compared with the Control (CON) group. (F) Immunofluorescence of HSPA8 in NIH3T3 cells. The green fluorescence indicates HSPA8 protein and the red fluorescence indicates mitochondria. Scale bar = 50μm. VDAC1: voltage-dependent anion channel 1, mitochondrial marker protein. (G) Cell mitochondria were isolated and HSPA8 protein expression was detected using Western blot. (H) Quantification of (G). * p < 0.05 compared with the CON group.