Table 1.
Target Gene | miRNA | Primer |
---|---|---|
ESR2 | cfa-miR-8881 | UUUGUUUUCUCUGGUUCUGUACC |
cfa-miR-8837 | UUCUUGCUGGAGUCCGGUUGUCU | |
cfa-let-7c | UGAGGUAGUAGGUUGUAUGGUU *** | |
FSH-R | cfa-miR-8900 | UAGGACUUUAAUGGCUGGAGAGA |
cfa-miR-34c | AGGCAGUGUAGUUAGCUGAUUGC | |
cfa-miR-34a | UGGCAGUGUCUUAGCUGGUUGU. *** | |
ref miRNAs | cfa-miR-16 | 5′-UAGCAGCACGUAAAUAUUGGCG-3′ |
ref miRNAs | cfa-miR-26 | 5′-UUCAAGUAAUCCAGGAUAGGCU-3′ |
*** Selected miRNA considering qPCR performance and target predicted probability with binding p-value of 1.0.