Table 2.
Sequences of primers for references genes used in this study and FSH-R, ESR2 genes evaluated for qRT-PCR analysis.
| Gene | Sequence 5′-3′ | Accession Number | Amplicon | Tm °C | Efficence |
|---|---|---|---|---|---|
| ACTB | F:ATTGTCATGGACTCTGGGGATG | AF021873.2 | 191 bp | 56.7 | 1.99 |
| R:TCCTTGATGTCACGCACGAT | |||||
| H2A | F:AGTACCTGACGGCCGAGAT | XM545419.4 | 245 bp | 59.6 | 1.97 |
| R:AGGGCAAATCAATCCAGAGA | |||||
| ESR2 | F: TTCTATAGCCCTGCTGTGATGAAT | AF389885.1 | 204 bp | 60.0 | 2.01 |
| R: ATTATGTCCTTGAATGCTTCTTT | |||||
| FSH-R | F:AACTCATTTGGCCATCCTTG | NC051814.1 | 212 bp | 60.0 | 1.98 |
| R:TGACTGCACCTTAGGCAGTG |
Abbreviation: FSHR, follicle-stimulating hormone gene receptor. ESR2, Estrogen gene receptor beta. ACTB, β-actin and H2A, histone genes.