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. 2023 Sep 12;325(5):G471–G491. doi: 10.1152/ajpgi.00090.2023

Figure 2.

Figure 2.

LPS/GalN-mediated induction of JNK signaling and hepatic inflammation is attenuated in P2Y2−/− mice. Total protein extracts of livers harvested at 1 h (A and B) and 5 h (C and D) post-LPS-GalN were analyzed by Western blotting with antibodies specific for phospho-JNK, total JNK, and β-actin (protein loading control; n, WT-Veh, 3, WT-LPS/GalN, 4; P2Y2-Veh, 3; P2Y2-LPS/GalN, 4). E–J: qRT-PCR analysis of total RNA isolated from the livers post-LPS/GalN at 1 h: TNFα, IL1β, MIP2; post-LPS/GalN at 5 h: MCP1, ICAM, iNOS (n, WT-Veh, 5, WT-LPS/GalN, 5; P2Y2-Veh, 7; P2Y2-LPS/GalN, 7–9). Bar diagrams represent relative expression. Means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. n, number of biological replicates. Veh, vehicle; WT, wild type.