Table 2.
Antibodies used for Western blotting
| Antibody | Source | Cat. No. | Primary Antibody Dilution | Secondary Antibody and Dilution |
|---|---|---|---|---|
| MMP-9 | Abcam | 38898 | 1:1,000 | GAR-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| Phospho-JNK (Thr183/Tyr185) | Cell Signaling Technology | 9251 | 1:1,000 | GAR-HRP1, 2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| JNK | Cell Signaling Technology | 9252 | 1:1,000 | GAR-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| Cleaved PARP-1 (Asp214) | Cell Signaling Technology | 9541 | 1:500 | GAR-HRP, 1:2,000 |
| TBST + 5% NFDM | TBST + 5% NFDM | |||
| Cleaved Caspase-3 (Asp175) | Cell Signaling Technology | 9661 | 1:500 | GAR-HRP, 1:2,000 |
| TBST + 5% NFDM | TBST + 5% NFDM | |||
| iNOS | Abcam | 15323 | 1:1,000 | GAR-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| NLRP3 (D4D8T) | Cell Signaling Technology | 15101 | 1:1,000 | GAR-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| α-Tubulin | Sigma | T9026 | 1:4,000 | HAM-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| β-Actin | Sigma | A5316 | 1:10,000 | HAM-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM | |||
| GAPDH | Cell Signaling Technology | 2118 | 1:4,000 | GAR-HRP, 1:2,000 |
| TBST + 5% BSA | TBST + 5% NFDM |
BSA, bovine serum albumin; GAR-HRP, goat anti-rabbit IgG, horseradish peroxidase-linked antibody; HAM-HRP, horse anti-mouse IgG, horseradish peroxidase-linked antibody; iNOS, inducible nitric oxide synthase; JNK, c-Jun NH2-terminal kinase; MMP-9, matrix metallopeptidase-9; NLRP3, nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3; NFDM, nonfat dry milk; TBST, tris-buffered saline.