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PCR amplification of fiber gene from field isolates. Lanes 1 to 8, Ad3 (strain YC 64-222), Ad3 (strain YC 64-227), Ad4 (strain YC 86-163), Ad7 (strain YC 95c-Bal), Ad8 (strain Miya-32), Ad19 (strain YC 85-80), Ad19 (strain YC 85-81), and Ad37 (strain YC 81-117), respectively; lane N, negative control; lane P, positive control (Ad19); lanes M, molecular size markers (100-bp DNA ladder; New England Biolabs). Values to the left and right of the gel are in base pairs.
