TABLE 1.

Primers used in the multiplex PCR for detection of tviB and SPI-7 in Vi agglutination-negative Salmonella isolates

Interpretation if band detected	Primer	Sequence	Positive control strain(s)	Product size (bp [gene amplified])
Genes for Vi production present	tviB-F	5′-CGAGTGAAACCGTTGGTACA-3′	SARB63, TY2, CT18	846 (tviB)
	tviB-R	5′-CAATGATCGCATCGTAGTGG-3′
SPI-7 absent	DE0032-F	5′-GCTCAGTCGGTAGAGCAGGGGATT-3′	SARB 64	1,275 (tRNA-phoN)
	DE0083-R	5′-TCATCTTCAGGACGGCAGGTAGAATG-3′
Salmonella DNA present	aroC-F	5′-GGCACCAGTATTGGCCTGCT-3′	CT18	639 (aroC)
	aroC-R	5′-CATATGCGCCACAATGTGTTG-3′