Table 1.
List of primary antibodies used in Western blotting.
| Primary Antibody |
Host | Dilution | Molecular Weight (kD) | Catalog Number |
Source |
|---|---|---|---|---|---|
| anti-IL6 | rabbit | 1:1000 | 24 | DF6087 | Affinity Biosciences, Cincinnati, OH, USA |
| anti-IL1β | rabbit | 1:1000 | 30 | AF5103 | Affinity Biosciences, Cincinnati, OH, USA |
| anti-TNFα | mouse | 1:500 | 26 | 60291-1-Ig | Proteintech, Rosemont, IL, USA |
| anti-iNOS | rabbit | 1:1000 | 130 | AF0199 | Affinity Biosciences, Cincinnati, OH, USA |
| anti-NFκB | rabbit | 1:1000 | 60 | 622601 | Biolegend, San Diego, CA, USA |
| anti-IκB | rabbit | 1:1000 | 36 | 10268-1-AP | Proteintech, Rosemont, IL, USA |
| anti-SOD2 | rabbit | 1:1000 | 22 | 13194 | Cell Signaling, Danvers, MA, USA |
| anti-CAT | mouse | 1:1000 | 60 | 863301 | Biolegend, San Diego, CA, USA |
| anti-Nrf2 | rabbit | 1:1000 | 68 | 16396-1-AP | Proteintech, Rosemont, IL, USA |
| anti-Keap1 | rabbit | 1:1000 | 70 | 10503-2-AP | Proteintech, Rosemont, IL, USA |
| anti-Sirt1 | mouse | 1:1000 | 120 | 8469 | Cell Signaling, Danvers, MA, USA |
| anti-PGC1ɑ | rabbit | 1:1000 | 91 | NBP1-04676 | Novus Biologicals, Centennial, CO, USA |
| anti-Nrf1 | rabbit | 1:1000 | 54 | AB175932 | Abcam, Cambridge, UK |
| anti-TFAM | rabbit | 1:1000 | 28 | AB131607 | Abcam, Cambridge, UK |
| anti-Caspase-3 | rabbit | 1:1000 | 32 | 19677-1-AP | Proteintech, Rosemont, IL, USA |
| anti-Bax | rabbit | 1:1000 | 21 | 50599-2-Ig | Proteintech, Rosemont, IL, USA |
| anti-Bcl2 | rabbit | 1:1000 | 26 | 26593-1-AP | Proteintech, Rosemont, IL, USA |
| anti-GFAP | rabbit | 1:1000 | 50 | DF6040 | Affinity Biosciences, Cincinnati, OH, USA |
| anti-GAPDH | rabbit | 1:1000 | 37 | 2118 | Novus Biologicals, Centennial, CO, USA |