FIG. 4.
Southern hybridization analysis of chromosomal DNA from wild-type A. hydrophila AG2 (lanes 2 and 3) and an aroA mutant (lanes 1 and 4). Total-cell DNA was BamHI digested and probed with the EcoRI-SmaI 0.7-kb fragment from aroA (lanes 1 and 2) or with the HincII 1.4-kb Kar cassette (lanes 3 and 4).