TABLE 1.
Characteristics of bacterial strains and plasmids used in this study
Strain or plasmid | Characteristic(s) | Source or reference |
---|---|---|
Strains | ||
E. coli AB2829 (aroA) | K-12 derivative with single mutation in aroA | 33 |
E. coli C600-1 | Transformation recipient for plasmids | 28 |
E. coli S17-1 | Mobilizing donor for conjugation | 42 |
A. hydrophila AG2 | Virulent rainbow trout isolate | 2a |
A. hydrophila aroA | aroA::Kar mutant | This study |
Plasmids | ||
pUC18 | Apr, cloning vector | 49 |
pJRD215 | Kar Smr, broad-host-range mobilizable vector | 37 |
pSUP202 | Tcr Apr Cmr, ColE1 ori, Mob+, broad-host-range mobilizable suicide vector | 42 |
pARO39 | A. hydrophila aroA gene cloned into pUC18 | This study |
pSARO39 | PvuII aroA-containing fragment from pARO39 cloned into pSUP202 | This study |
pULMJ8 | Apr Kar Cmr, source of the 1.4-kb HincII Kar-containing fragment | 12 |
pSKARO239 | 1.4-kb HincII Kar fragment from pULMJ8 cloned into the SmaI site of pSUP-ARO39 | This study |