TABLE 2.
Mortalities recorded for LD50 calculations of the AG2, aroA mutant, and AroA+ strains of A. hydrophila
No. of bacteria/0.1 ml | No. of fish that died after inoculation witha:
|
|||||
---|---|---|---|---|---|---|
AG2
|
aroA mutant
|
AroA+b
|
||||
Expt 1 | Expt 2 | Expt 1 | Expt 2 | Expt 1 | Expt 2 | |
109 | 10 | 10 | 6 | 5 | 10 | 10 |
108 | 9 | 10 | 4 | 4 | 8 | 9 |
107 | 8 | 8 | 2 | 2 | 8 | 8 |
106 | 6 | 6 | 0 | 0 | 6 | 5 |
105 | 3 | 3 | 0 | 0 | 3 | 3 |
104 | 0 | 0 | 0 | 0 | 0 | 0 |
103 | 0 | 0 | 0 | 0 | 0 | 0 |
LD50 | 7 × 105 | 6 × 105 | 2 × 108 | 2 × 108 | 8 × 105 | 1 × 106 |
10 fish per group were inoculated.
Reverted strain (aroA::Kar complemented with the wild-type aroA gene cloned into pJRD215).