FIG. 7.
R. rickettsii-induced NF-κB activation in cytoplasmic extracts of T24 bladder carcinoma cells transfected with either empty LXSN vector (T24cyt) or IκBαM-containing vector (MutT24cyt). Cytoplasmic extracts (20-μl aliquots) were incubated alone or in the presence of 4 × 105 PFU of partially purified R. rickettsii (+RR) for 30 min prior to assay. Preparation of R. rickettsii (RR) was also analyzed to control for the presence of any preexisting activated NF-κB species. NS, nonspecific complex.