TABLE 2.
Comparative sensitivity for virus detection in organs of experimentally infected chickens by LAMP and nested PCR
| Specimen | 3 days after inoculation
|
6 days after inoculation
|
||
|---|---|---|---|---|
| LAMP | Nested PCR | LAMP | Nested PCR | |
| Brain | − | − | − | − |
| Trachea | + (3/4) | + (3/4) | + (4/4) | + (4/4) |
| Lung | + (3/4) | + (3/4) | + (4/4) | + (4/4) |
| Kidney | − | − | − | − |
| Spleen | − | − | − | − |
| Liver | − | − | − | − |
| Pancreas | − | − | − | − |
| Intestine | − | − | − | − |
| Cecal tonsil | − | − | − | − |
| Bursa | − | − | − | − |
−, negative; +, positive. The number positive out of the number tested is given in parentheses.