TABLE 1.
DNA probes hybridizing to colony blots of diffusely adherent E. coli isolates
| Probe | Description of probe | Properties of probe (reference) | No. (%) of DAEC strains isolated from:
|
||
|---|---|---|---|---|---|
| Cases (n = 61) | Controls (n = 51) | Cases and controls (total) (n = 112) | |||
| daaE | 419-bp amplified fragment | F1845 fimbrial subunit (7) | 6 (9.8) | 9 (17.6) | 15 (13.4) |
| afa | 750-bp amplified fragment | Afimbrial adhesin (16) | 9 (14.7) | 8 (15.7) | 17 (15.2) |
| aggA | 450-bp amplified fragment | AAF/I fimbrial subunit (8) | 1 (1.6) | 0 | 1 (0.9) |
| aafA | 550-bp amplified fragment | AAF/II fimbrial subunit (8) | 0 | 0 | 0 |
| agg-3A | 485-bp amplified fragment | AAF/III fimbrial subunit (5) | 10 (16.4) | 10 (19.6) | 20 (17.8) |
| fimH | 527-bp amplified fragment | Type 1 fimbriae (14) | 26 (42.6) | 28 (54.9) | 54 (48.2) |
| sfa | 410-bp amplified fragment | S fimbriae (16) | 1 (1.6) | 2 (3.9) | 3 (2.7) |
| pap | 328-bp amplified fragment | P fimbriae (16) | 3 (4.9) | 3 (5.9) | 6 (5.3) |
| iucA | 1,100-bp amplified fragment | Aerobactin (20) | 31 (50.8) | 25 (49) | 56 (50) |
| hly | 7,000-bp fragment of pSF4000 | Alpha-hemolysin (29) | 4 (6.5) | 2 (3.9) | 6 (5.4) |
| pet | 1,037-bp amplified fragment | 104-kDa cytotoxin (28) | 0 | 0 | 0 |
| astA | 111-bp amplified fragment | EAST1 toxin (30) | 11 (18) | 6 (11.8) | 17 (15.2) |
| shET1 | 309-bp amplified fragment | Shigella enterotoxin 1 (28) | 7 (11.5) | 1 (1.9) | 8 (7.1) |
| shET2 | 799-bp amplified fragment | Shigella enterotoxin 2 (28) | 0 | 0 | 0 |
| pic | 5,800-bp amplified fragment | Mucinase (28) | 0 | 0 | 0 |
| cnf | 498-bp amplified fragment | Cytotoxic necrotizing factor (22) | 1 (1.6) | 1 (1.9) | 2 (1.8) |
| cdt | 175-bp fragment of pCVD448 | Cytolethal distending toxin (26) | 0 | 0 | 0 |
| espC | 2,000-bp fragment of pMS1 | Enterotoxin (27) | 0 | 0 | 0 |