TABLE 1.
Recovery and consistency of DNA isolation from DBS and whole blooda
| Specimen | No. of samples | Avg CTb
|
|
|---|---|---|---|
| HIV LTR | RNaseP | ||
| Whole blood | 4 | 34.0 (0.25) | 22.4 (0.26) |
| DBS | 4 | 33.6 (0.09) | 21.6 (0.35) |
a
Venous blood from a seronegative person was spiked with 8E5 cells, and a DBS was prepared. DNA were extracted from the DBS (one circle = 50 μl of blood) and the blood (50 μl) directly in quadruplicate. The extraction procedure of whole-blood DNA was the same as that for its DBS counterpart but without the use of DBS extraction buffer and 90°C incubation. In both cases, DNA was recovered in 50 μl of water, and 20 μl was used in the duplex assay.
b
One standard deviation is given in parentheses.