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. 2024 Jan 19;12(1):224. doi: 10.3390/biomedicines12010224

Figure 5.

Figure 5

Cytotoxicity and proinflammatory cytokine production in response to silicone implantation in 3D skin equivalent. (a) Culture supernatants were used for measuring cytotoxicity via lactate dehydrogenase assay on days 5, 10, 15, 20, 25, and 30 after 3D skin equivalent production. (b–g) The concentrations of IL-1α (b), IL-6 (c), IL-8 (d), IL-33 (e), MCP-1 (f), and TNF-α (g) in culture supernatant were quantified with ELISA on day 30 of cultures. The graphs represent the mean values ± SD from at least three independent replicates. Statistical significance of the changes in cytokine concentration in comparison to control without implant was tested with the t-test: * p ≤ 0.005, ** p ≤ 0.001.