Table 1.
Materials and applications to clarify the pathophysiology of CADASIL.
| Human materials: autopsied brain tissue or vessels, skin biopsy specimens | |
| Histopathology, immunohistochemistry | |
| Biochemistry, proteomics | |
| Gene expression, transcriptome | |
| Animal models: transgenic mice | |
| Genetic approach to clarify pathophysiology | |
| Temporal analysis of disease process | |
| Histopathology, immunohistochemistry | |
| Biochemistry, proteomics | |
| Gene expression, transcriptome | |
| Cell cultures: VSMCs 1, iPS cell-derived mural cells, cell lines (HEK293, NIH 3T3, etc.) | |
| Analysis of Notch signaling activity | |
| Recreation of pathology of CADASIL | |
| Vaiability and proliferation | |
| In vitro | |
| Aggregation assay of N3ECDpeptides | |
1 vascular smooth muscle cells.