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. 2005 Apr;43(4):1982–1984. doi: 10.1128/JCM.43.4.1982-1984.2005

FIG. 1.

FIG. 1.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (A) and Western blot analysis (B) of recombinant nucleocapsid protein of M41 IBV expressed in S. cerevisiae. Lanes: 1, molecular size marker; 2, proteins of M41 IBV; 3, recombinant IBV N protein purified by nickel-agarose column chromatography; 4, crude protein extract of transformed S. cerevisiae. Arrows indicate the natural IBV N protein (a) and yeast-expressed recombinant N protein (b).