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. 2024 Jan 22;12(1):246. doi: 10.3390/biomedicines12010246

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Levels of PAR2 and PAR4 as well as stem cell markers in various ovarian cancer cell lines. Preparation of sh-Par2/f2rl1-GFP. Characterization of knockdown PAR2 ES2 cells. (A) RT-PCR analysis of stem cell markers. ES2, OVCAR3, and OVCAR8 ovarian cancer cell lines were evaluated for levels of stem cell markers. GAPDH served as a housekeeping gene. (B) ES2 metastatic ovarian cells were infected with a lentivirus containing either Par2-shRNA cassette or scrambled shRNA cassette. GFP fluorescence indicated the infection efficiency. More than 90% of the cells showed marked expression of GFP, indicating effective infection. (C) Clones of Par2-shRNA effectively inhibit hPar2 mRNA levels in ES2 cells. GAPDH served as a housekeeping gene.