Figure 4.

PAR2 forms a complex with PH-signal proteins in ES2 and OVCAR3 ovarian cancer cell lines. (a) ES2 ovarian carcinoma cells were activated with SLIGKV for 5′, 15′, and 30′. Application of anti-PAR2 abs was carried out on ES2 cell lysates to immunoprecipitate endogenous levels of PAR2. Akt was detected following Western blotting with anti-Akt abs. Increased association between PAR2 and Akt was observed 10 min after SLIGKV activation. (b) OVCAR3 cells were activated with SLIGKV for the indicated periods of time. Cell lysates were immunoprecipitated with anti-PAR2 Abs. Etk/Bmx was detected following Western blotting with anti-Etk/Bmx abs. Levels of Etk/Bmx are shown per equal PAR2 protein immunoprecipitated.