TABLE 2.
Detection of influenza A and influenza B viruses and adenovirus in 2002-2003 nasal swab specimens stored in ethanol at ambient temperature by TM and LightCycler SG PCR methodsa
| Detection method | Mo no. | Sensitivity
|
Specificity
|
PPV
|
NPV
|
||||
|---|---|---|---|---|---|---|---|---|---|
| No. of culture positive specimens/ total no. of specimens | % of specimens culture positive (95% CI) | No. of culture positive specimens/ total no. of specimens | % of specimens culture positive (95% CI) | No. of culture positive specimens/ total no. of specimens | % of specimens culture positive | No. of culture positive specimens/ total no. of specimens | % of specimens culture positive | ||
| Influenza A virus (12 TP) | |||||||||
| Viral culture | 1 | 11/12 | 92 (76-100) | 177/177 | 100 | 11/11 | 100 | 177/178 | 99 |
| Primary TM | 1 | 9/12 | 75 (51-100) | 174/177 | 98 (96-100) | 9/12 | 75 | 174/177 | 98 |
| 6 | 8/12 | 67 (40-93) | nd | 8/12 | 67 | nd | |||
| Secondary SG | 1 | 7/12 | 58 (30-86) | 177/177 | 100 | 7/7 | 100 | 177/182 | 97 |
| 6 | 8/12 | 67 (40-93) | nd | 8/8 | 100 | nd | |||
| Influenza B virus (9 TP) | |||||||||
| Viral culture | 1 | 7/9 | 78 (51-100) | 180/180 | 100 | 7/7 | 100 | 180/182 | 99 |
| Primary TM | 1 | 8/9 | 89 (68-100) | 180/180 | 100 | 8/8 | 100 | 180/181 | 99 |
| 6 | 8/9 | 89 (68-100) | nd | 8/8 | 100 | nd | |||
| Secondary SG | 1 | 6/9 | 67 (36-97) | 180/180 | 100 | 6/6 | 100 | 180/183 | 98 |
| 6 | 9/9 | 100 | nd | 9/9 | 100 | nd | |||
| Adenovirus (122 TP) | |||||||||
| Viral culture | 63/122 | 52 (43-60) | 67/67 | 100 | 63/63 | 100 | 67/126 | 53 | |
| Primary SG | 1 | 69/122 | 57 (48-65) | 64/67 | 96 (91-100) | 69/72 | 96 | 64/117 | 55 |
| Primary TM | 1 | 119/122 | 98 (95-100) | 47/67 | 70 (59-81) | 119/139 | 86 | 47/50 | 94 |
| Secondary TM | 1 | 113/122 | 93 (88-97) | 59/67 | 88 (80-96) | 113/121 | 93 | 59/68 | 87 |
a
TP, true positive specimen (positive by culture and/or two molecular tests); CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; nd, not determined. Specificity and PPV of viral culture are 100% by default, due to the definition of “true positive.”